Fluorescent-marked protein is a useful asset for the identification of CAR articulation in research and clinical examples by stream cytometry. These proteins are pre-marked fluorescent colors that can identify CAR articulation by one-venture staining with insignificant foundation. Notwithstanding, the advancement of excellent fluorescent-named proteins presents a significant test and there are just couple of items industrially accessible up until this point.
Formation strategy is a significant bottleneck for growing excellent fluorescent-marked proteins. At present, the most generally involved naming strategy in the market is the conventional substance marking approach. It is an extremely simple strategy to name protein with a fluorescent color in a vague way covalently.
In any case, arbitrary adjustment can prompt heterogeneous and may influence the bioactivity of the protein by means of obstructing the protein-dynamic site. Studies demonstrate that the new-age site-explicit marking innovation can append fluorescent colors to explicit protein in site-explicit way. It gives an assurance to the consistency of the fluorescent-marked items and tries not to influence protein movement. This property makes it a valuable weapon for the improvement of great fluorescent-named protein items with high explicitness and awareness.
ACROBiosystems is taking a stab at fostering the elevated requirement recognition reagents to help the advancement of CAR-T cell treatment. Following quite a while of examination and experience by our specialists, we effectively settled the “Star Staining” site-explicit naming stage and fostered a progression of restrictive fluorescent-marked proteins with “Star Standard” quality. “Star Staining” items can be the incredible assets for identifying and observing CAR-T cells in clinical preliminaries.
Utilizing new-age site-explicit naming innovation to keep up with normal bioactivity
- High particularity and awareness confirmed by stream cytometry
- No vague restricting to non-transduced PBMCs
- High cluster to-bunch consistency and consistency.
Item Feature
- Customary Chemical Labeling
- Non-particular arbitrary marking and influence regular design
- May cover the dynamic locales to lessen bioactivity
- Simple to total because of progress the hydrophilicity of proteins
- High variety in clump to-group consistency
New-age Site-explicit Labeling
- The marking occurs at the particular tag and distant from dynamic locales of proteins
- Keep up with normal compliance and adjustment
- Effectively bio-symmetrical innovation
- High clump to-bunch consistency and consistency.
Apoptosis
Apoptosis is a perplexing and dynamic demise, including the initiation, articulation and guideline of a progression of qualities. Whenever apoptosis happens, a progression of apoptotic processes are modified, for example, phosphatidylserines valgus beyond the cell film, mitochondrial layer Δψm changes, caspase initiation, DNA discontinuity, etc. Unusual apoptotic system can make cells let completely go, which is firmly connected with the event and advancement of numerous infections like growths. Exact identification of apoptotic cells is fundamental in the apoptosis study. Elabscience has effectively fostered different apoptosis identification items (Annexin V Detection Kits, TUNEL Assay Kits,etc) in view of the most generally utilized standards.
Apoptosis Detection Kit.
MCE Annexin V-FITC/PI Apoptosis Detection Kit gives a quick and advantageous technique to recognize cell apoptosis and corruption. In typical live cells, Phosphatidylserine (PS) is situated on the cytoplasmic surface of the cell film. Endless supply of apoptosis, PS moves from the internal to the external pamphlet of the film. Annexin V is a 35-36 kDa Ca2+-subordinate phospholipid-restricting protein that has a high liking for PS.
Annexin V (FITC) | ||||
| abx090611-100tests | Abbexa | 100 tests | 510 EUR | |
Annexin V FITC | ||||
| EXB0024 | ExBio | 100 tests | 244.8 EUR | |
Annexin V, FITC Labeled | ||||
| 20030 | AAT Bioquest | 100 tests | 262.8 EUR | |
Annexin V, FITC conjugate | ||||
| 29001 | Biotium | 0.5ML | 332.4 EUR | |
Annexin V-FITC Reagent | ||||
| 1001-1000 | Biovision | each | 1495.2 EUR | |
Annexin V-FITC Reagent | ||||
| 1001-200 | Biovision | each | 529.2 EUR | |
Annexin A4 (ANXA4) Monoclonal Antibody (Human), FITC | ||||
| 4-MAE784Hu22-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Monoclonal Antibody (Pig), FITC | ||||
| 4-MAE784Po21-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Monoclonal Antibody (Rat), FITC | ||||
| 4-MAE784Ra21-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Monoclonal Antibody (Human), FITC | ||||
| 4-MAE786Hu21-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Monoclonal Antibody (Human), FITC | ||||
| 4-MAE786Hu23-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Monoclonal Antibody (Dog), FITC | ||||
| 4-MAE787Ca21-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Monoclonal Antibody (Rat), FITC | ||||
| 4-MAE787Ra21-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Monoclonal Antibody (Human), FITC | ||||
| 4-MAA259Hu22-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAA259Mu01-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Polyclonal Antibody (Rat), FITC | ||||
| 4-PAA259Ra01-FITC | Cloud-Clone |
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Annexin A9 (ANXA9) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE779Hu01-FITC | Cloud-Clone |
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Annexin A10 (ANXA10) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE780Hu01-FITC | Cloud-Clone |
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Annexin A13 (ANXA13) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE783Hu01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Bovine), FITC | ||||
| 4-PAE784Bo01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE784Hu01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAE784Mu01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Pig), FITC | ||||
| 4-PAE784Po01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Pig), FITC | ||||
| 4-PAE784Po02-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Rat), FITC | ||||
| 4-PAE784Ra01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Rat), FITC | ||||
| 4-PAE784Ra02-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE786Hu01-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE786Hu02-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAE786Mu01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Polyclonal Antibody (Human), FITC | ||||
| 4-PAE787Hu01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAE787Mu01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Polyclonal Antibody (Pig), FITC | ||||
| 4-PAE787Po01-FITC | Cloud-Clone |
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Annexin A6 (ANXA6) Polyclonal Antibody (Human), FITC | ||||
| 4-PAC345Hu01-FITC | Cloud-Clone |
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Secretogranin V (SCG5) Polyclonal Antibody (Human), FITC | ||||
| 4-PAC834Hu01-FITC | Cloud-Clone |
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Cathepsin V (CTSV) Polyclonal Antibody (Human), FITC | ||||
| 4-PAC970Hu01-FITC | Cloud-Clone |
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Annexin V (ANXA5) Antibody (FITC) | ||||
| abx414513-100tests | Abbexa | 100 tests | 610.8 EUR | |
Annexin V-FITC Apoptosis Kit | ||||
| K2003-25 | ApexBio | 25 assays | 285.6 EUR | |
Annexin V-FITC Apoptosis Kit | ||||
| K2003-400 | ApexBio | 400 assays | 1336.8 EUR | |
Annexin V-FITC Apoptosis Kit | ||||
| K101-100 | Biovision | each | 614.4 EUR | |
Annexin V-FITC Apoptosis Kit | ||||
| K101-25 | Biovision | each | 268.8 EUR | |
Annexin V-FITC Apoptosis Kit | ||||
| K101-400 | Biovision | each | 1227.6 EUR | |
Annexin A2 (ANXA2) Polyclonal Antibody (Human, Rat), FITC | ||||
| 4-PAB944Hu01-FITC | Cloud-Clone |
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Annexin A2 (ANXA2) Polyclonal Antibody (Human, Mouse), FITC | ||||
| 4-PAB944Mu01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Monoclonal Antibody (Guinea pig), FITC | ||||
| 4-MAE787Gu21-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Monoclonal Antibody (Human, Mouse), FITC | ||||
| 4-MAE787Hu22-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Polyclonal Antibody (Human, Bovine), FITC | ||||
| 4-PAA259Bo01-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Polyclonal Antibody (Human, Chicken), FITC | ||||
| 4-PAA259Ga01-FITC | Cloud-Clone |
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Annexin A5 (ANXA5) Polyclonal Antibody (Human, Mouse), FITC | ||||
| 4-PAA259Hu01-FITC | Cloud-Clone |
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Annexin A11 (ANXA11) Polyclonal Antibody (Human, Mouse), FITC | ||||
| 4-PAE781Hu01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Rat, Dog), FITC | ||||
| 4-PAE784Ca01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Rat, Dog), FITC | ||||
| 4-PAE784Ca02-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Mouse, Rat), FITC | ||||
| 4-PAE784Mu02-FITC | Cloud-Clone |
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Annexin A3 (ANXA3) Polyclonal Antibody (Human, Rat), FITC | ||||
| 4-PAE786Ra01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Polyclonal Antibody (Guinea pig), FITC | ||||
| 4-PAE787Gu01-FITC | Cloud-Clone |
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Annexin A1 (ANXA1) Polyclonal Antibody (Mouse, Rat), FITC | ||||
| 4-PAE787Ra01-FITC | Cloud-Clone |
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Annexin A6 (ANXA6) Polyclonal Antibody (Human, Mouse), FITC | ||||
| 4-PAC345Mu01-FITC | Cloud-Clone |
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Integrin Alpha V (ITGaV) Polyclonal Antibody (Human), FITC | ||||
| 4-PAB282Hu01-FITC | Cloud-Clone |
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Glycoprotein V, Platelet (GP5) Polyclonal Antibody (Human), FITC | ||||
| 4-PAB300Hu01-FITC | Cloud-Clone |
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Glycoprotein V, Platelet (GP5) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAB300Mu01-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Bovine), FITC | ||||
| 4-PAA675Bo01-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Bovine), FITC | ||||
| 4-PAA675Bo02-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Human), FITC | ||||
| 4-PAA675Hu01-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Human), FITC | ||||
| 4-PAA675Hu02-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Human), FITC | ||||
| 4-PAA675Hu03-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAA675Mu01-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAA675Mu02-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAA675Mu03-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Pig), FITC | ||||
| 4-PAA675Po01-FITC | Cloud-Clone |
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Coagulation Factor V (F5) Polyclonal Antibody (Rat), FITC | ||||
| 4-PAA675Ra01-FITC | Cloud-Clone |
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Annexin A4 (ANXA4) Polyclonal Antibody (Human, Mouse, Rat), FITC | ||||
| 4-PAE784Hu02-FITC | Cloud-Clone |
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Integrin Alpha V (ITGaV) Polyclonal Antibody (Human, Mouse), FITC | ||||
| 4-PAB282Mu01-FITC | Cloud-Clone |
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Annexin V Apoptosis Detection Kit (FITC) | ||||
| 55R-1265 | Fitzgerald | 25 assays | 332.4 EUR | |
Annexin V Apoptosis Detection Kit (FITC) | ||||
| abx290002-100test | Abbexa | 100 test | 644.4 EUR | |
Annexin V Apoptosis Detection Kit (FITC) | ||||
| abx290014-200test | Abbexa | 200 test | 577.2 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K2057-100 | ApexBio | 100 assays | 619.2 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K2057-25 | ApexBio | 25 assays | 301.2 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K2057-400 | ApexBio | 400 assays | 1270.8 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K201-100 | Biovision | each | 601.2 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K201-25 | Biovision | each | 288 EUR | |
Annexin V-FITC Apoptosis Kit Plus | ||||
| K201-400 | Biovision | each | 1136.4 EUR | |
Collagen Type V Alpha 2 (COL5a2) Polyclonal Antibody (Human), FITC | ||||
| 4-PAC152Hu01-FITC | Cloud-Clone |
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H2A Histone Family, Member V (H2AFV) Polyclonal Antibody (Human), FITC | ||||
| 4-PAJ708Hu01-FITC | Cloud-Clone |
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V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) Polyclonal Antibody (Human), FITC | ||||
| 4-PAB290Hu01-FITC | Cloud-Clone |
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V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) Polyclonal Antibody (Mouse), FITC | ||||
| 4-PAB290Mu01-FITC | Cloud-Clone |
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Annexin V named with green fluorescent FITC can recognize apoptotic cells by restricting to PS uncovered on the external flyer. Propidium Iodide (PI) is a cell-film impermeable color to live cells and early apoptosis cells, yet stains late apoptosis cells and corruption cells with red fluorescence. In the wake of staining, live cells show practically no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and corruption cells show red and green fluorescence (Annexin V+/PI+).