Skip to content

Entigen Corporation San Jose, CA

Microarray Signarray mRNA library, cancer array

Menu
  • Home
  • Product
  • Elisa Kits
  • DNA
  • Distributors
  • Blog
Menu

Apo Active PE- Caspase 3

Posted on April 23, 2022 by Zachary

Caspase (Cysteine-requiring Aspartate Protease) is a protease family that assumes a significant part during the time spent apoptosis. Caspase 3 (otherwise called CPP32, Yama, or apopain) is an individual from the CED-3 subfamily of Caspases and is one of the basic chemicals of apoptosis. Caspase 3 is the most concentrated on Caspase in mammalian cells.

Caspase 3 can be utilized to cut proCaspase2, 6, 7, 9, and cut explicitly many substrates of Caspase straightforwardly, including PPAR (poly ADP-ribose), the inhibitor of Caspase-actuated deoxyribonuclease (ICAD), gelsolin and fodrin. Protein shearing intervened by Caspase 3 are significant pieces of the sub-atomic system of apoptosis.

Furthermore, Caspase 3 likewise assumes a critical part during the time spent atomic apoptosis including chromatin buildup and DNA fracture as well as cell blebbing. Caspase 3 exists as prozyme in the typical state and has no movement. Nonetheless, during the apoptosis stage, initiated Caspase 3 comprises by two enormous subunits and two little subunits, which cleavages the relating substrate of endochylema or cytoplasmic atomic and in the end prompts apoptosis.

This pack is utilized to form Caspase 3 succession explicit peptides acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) to yellow gathering p-nitroaniline (pNA). At the point when the substrate is cut by Caspase 3, the yellow gathering pNA is separated. pNA has an assimilation top at 405 nm. Measure the OD esteem at 405 nm and afterward Caspase 3 action can be determined likewise.

What are caspases?

Caspases are a group of cysteine-aspartic corrosive explicit proteases (CED-3/ICE) that have been recognized as significant go betweens of the complex biochemical occasions related with apoptosis .

Caspases are isolated into two gatherings: initiator and effector caspases, and when actuated by the initiator caspase (for example caspase-8 and – 9), the effector caspases (for example caspases-3 and – 7) capacity to sever protein substrates and start apoptosis .

The acknowledgment site for every caspase is set apart by three to four amino acids followed by an aspartic corrosive buildup, with the cleavage happening after the aspartate. The caspase proteases are commonly integrated as dormant forerunners and inhibitor delivery or cofactor restricting enacts the caspase through cleavage at inward aspartates, either via autocatalysis or by the activity of another protease.

Not all caspases are associated with apoptosis and lately, it has been exhibited that a few caspases might be engaged with irritation . Since cell passing fountains are mind boggling and dynamic, it is enthusiastically suggested that a multi-parametric methodology is directed for a precise evaluation of apoptosis.

Apo Active 3 FITC (FAB200)

Exceptionally unambiguous for dynamic human and murine caspase 3. Different measures require the usage of peptide based (DEVD) reagents that will quite often cross-respond with caspase 7 and different caspases.
Applications – Works with a fluorescence magnifying instrument, 96 well plate peruser, or stream cytometer. Yields both quantitative and subjective outcomes. Gives a solid positive sign.
Can be utilized related to different antibodies or stains.
Don’t bother making cell lysates or run western smudges. Cells can be fixed and examined later.
Works with human, mouse and rodent cell lines.

Acceptance of caspase 3 actuation by numerous L. pneumophila Dot/Icm substrates

The intracellular microbe Legionella pneumophila can find some kind of harmony between the demise and endurance of the host cell during contamination. Regardless of the presence of elevated degree of dynamic caspase-3, the killer caspase of apoptotic cell demise, tainted tolerant macrophages are uniquely impervious to exogenous apoptotic upgrades. A few bacterial atoms equipped for advancing the cell endurance pathways have been distinguished, yet proteins associated with the actuation of caspase-3 stay obscure. To concentrate on the component of L. pneumophila-interceded caspase-3 actuation, we tried all known Dot/Icm substrates for their capacity to enact caspase-3. Five effectors fit for causing caspase-3 enactment upon transient articulation were distinguished.

Telomerase Reverse Transcriptase Rabbit pAb

E2382962 EnoGene 100ul 225 EUR

Telomerase Reverse Transcriptase Rabbit pAb

E2160502 EnoGene 100ul 225 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064198-01mL MyBiosource 0.1mL 265 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064198-02mL MyBiosource 0.2mL 355 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064198-05mL MyBiosource 0.5mL 660 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064198-1mL MyBiosource 1mL 810 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064198-5mL MyBiosource 5mL 2330 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064204-01mL MyBiosource 0.1mL 275 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064204-02mL MyBiosource 0.2mL 365 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064204-05mL MyBiosource 0.5mL 675 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064204-1mL MyBiosource 1mL 835 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064204-5mL MyBiosource 5mL 2395 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064210-01mL MyBiosource 0.1mL 285 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064210-02mL MyBiosource 0.2mL 380 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064210-05mL MyBiosource 0.5mL 710 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064210-1mL MyBiosource 1mL 875 EUR

APC-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064210-5mL MyBiosource 5mL 2525 EUR

Telomerase reverse transcriptase

AP88990 SAB 1mg 2640 EUR

Telomerase reverse transcriptase

AP89047 SAB 1mg 2640 EUR

Telomerase reverse transcriptase

AP79299 SAB 1mg 2640 EUR

Telomerase reverse transcriptase

AP88607 SAB 1mg 2640 EUR

Telomerase reverse transcriptase

AP88646 SAB 1mg 2640 EUR

Telomerase reverse transcriptase

E8ET1702-22 EnoGene 100ul 275 EUR

Telomerase reverse transcriptase

MBS8534331-01mL MyBiosource 0.1mL 345 EUR

Telomerase reverse transcriptase

MBS8534331-01mLAF405L MyBiosource 0.1mL(AF405L) 565 EUR

Telomerase reverse transcriptase

MBS8534331-01mLAF405S MyBiosource 0.1mL(AF405S) 565 EUR

Telomerase reverse transcriptase

MBS8534331-01mLAF610 MyBiosource 0.1mL(AF610) 565 EUR

Telomerase reverse transcriptase

MBS8534331-01mLAF635 MyBiosource 0.1mL(AF635) 565 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064196-1mL MyBiosource 1mL 1185 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064196-5mL MyBiosource 5mL 3445 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064196-5x5mL MyBiosource 5x5mL 15495 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064202-1mL MyBiosource 1mL 1220 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064202-5mL MyBiosource 5mL 3540 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064202-5x5mL MyBiosource 5x5mL 15930 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064208-1mL MyBiosource 1mL 1285 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064208-5mL MyBiosource 5mL 3735 EUR

APC/CY7-Linked Polyclonal Antibody to Telomerase Reverse Transcriptase (TERT)

MBS2064208-5x5mL MyBiosource 5x5mL 16805 EUR

Telomerase reverse transcriptase Antibody

49336 SAB 100ul 499 EUR

Telomerase reverse transcriptase Antibody

49336-100ul SAB 100ul 399.6 EUR

Telomerase reverse transcriptase Antibody

49336-50ul SAB 50ul 286.8 EUR

Telomerase Reverse Transcriptase Antibody

MBS9436082-005mL MyBiosource 0.05mL 325 EUR

Telomerase Reverse Transcriptase Antibody

MBS9436082-01mL MyBiosource 0.1mL 435 EUR

Telomerase Reverse Transcriptase Antibody

MBS9436082-5x01mL MyBiosource 5x0.1mL 1810 EUR

Telomerase Reverse Transcriptase (TERT) Polyclonal Antibody (Rat), APC

4-PAC241Ra01-APC Cloud-Clone
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml

Telomerase Reverse Transcriptase Mouse mAb

E2222764 EnoGene 100ul 255 EUR

Human Telomerase reverse transcriptase (TERT)

1-CSB-EP023391HU Cusabio
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10ug
  • 50ug
  • 100ug
  • 200ug
  • 500ug
  • 1MG

Mouse Telomerase reverse transcriptase (Tert)

1-CSB-EP023391MO Cusabio
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10ug
  • 50ug
  • 100ug
  • 200ug
  • 500ug
  • 1MG

Telomerase Reverse Transcriptase Rabbit mAb

E2R382962 EnoGene 100ul 275 EUR

Telomerase Reverse Transcriptase (TERT) Polyclonal Antibody (Human), APC

4-PAC241Hu01-APC Cloud-Clone
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml

Telomerase Reverse Transcriptase (TERT) Polyclonal Antibody (Mouse), APC

4-PAC241Mu01-APC Cloud-Clone
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml

Telomerase reverse transcriptase Antibody (HRP)

20-abx108799 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase Reverse Transcriptase (TERT) Antibody

abx002167-100l Abbexa 100 µl 400 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx002167 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ul
  • 50 ul
  • 100 ul
  • 200 ul

Telomerase Reverse Transcriptase (TERT) Antibody

abx002167-20l Abbexa 20 µl 175 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx002167-50l Abbexa 50 µl 275 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027802-400l Abbexa 400 µl 518.75 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027802-400ul Abbexa 400 ul 627.6 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027802-80l Abbexa 80 µl 281.25 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027803-400l Abbexa 400 µl 518.75 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027803-400ul Abbexa 400 ul 627.6 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx027803-80l Abbexa 80 µl 281.25 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx016155-100ug Abbexa 100 ug 493.2 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx016155-1mg Abbexa 1 mg 362.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103374-100g Abbexa 100 µg 750 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx103374 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase Reverse Transcriptase (TERT) Antibody

abx103374-20g Abbexa 20 µg 275 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103374-50g Abbexa 50 µg 337.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103375-100g Abbexa 100 µg 775 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx103375 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase Reverse Transcriptase (TERT) Antibody

abx103375-20g Abbexa 20 µg 275 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103375-50g Abbexa 50 µg 350 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103376-100g Abbexa 100 µg 812.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx103376 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase Reverse Transcriptase (TERT) Antibody

abx103376-20g Abbexa 20 µg 287.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx103376-50g Abbexa 50 µg 362.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx110254-100l Abbexa 100 µl 162.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx110254 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase reverse transcriptase Antibody (FITC)

20-abx107379 Abbexa
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Telomerase Reverse Transcriptase (TERT) Antibody

abx146885-100ug Abbexa 100 ug 526.8 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx147263-100ug Abbexa 100 ug 526.8 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx339525-100g Abbexa 100 µg Ask for price

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx339525 Abbexa
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul

Telomerase Reverse Transcriptase (TERT) Antibody

abx339525-20g Abbexa 20 µg 250 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx339525-50g Abbexa 50 µg 350 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx433353-100g Abbexa 100 µg 387.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx433353-200ul Abbexa 200 ul 460.8 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx324977-100g Abbexa 100 µg 250 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

20-abx324977 Abbexa
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug

Telomerase Reverse Transcriptase (TERT) Antibody

abx324977-50g Abbexa 50 µg 187.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx224167-100g Abbexa 100 µg 362.5 EUR

Telomerase Reverse Transcriptase (TERT) Antibody

abx224167-100ug Abbexa 100 ug 493.2 EUR
×

Among these, by utilizing its capacity to initiate caspase-3 by instigating the arrival of cytochrome c from the mitochondria, we showed that VipD is a phospholipase A2, which hydrolyzes phosphatidylethanolamine (PE) and phosphocholine (PC) on the mitochondrial film in a way that seems to require have co-factor(s). The lipase movement prompts the creation of free unsaturated fats and 2-lysophospholipids, which undermine the mitochondrial film and may add to the arrival of cytochrome c and the ensuing caspase 3 actuation. Moreover, we viewed that as while it isn’t distinguishably imperfectly in caspase 3 enactment in tolerant cells, a freak coming up short on these five qualities is less intense in initiating apoptosis in dendritic cells. Our outcomes uncover that initiation of host cell demise pathways by L. pneumophila is a consequence of the impacts of numerous bacterial proteins with assorted biochemical capacities.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • Innovations in Glycosylation Mechanisms: Defining the Future of Biomanufacturing and Therapeutic Development
  • Glycan-Based Therapeutics: Exploring the Potential of Glycoscience in Advanced Medical Treatment
  • Utilizing the potential of Glycan-Protein Binding: An advanced method in Vaccine Engineering
  • Advancements in Glycobiology: Crafting Personalized Medicine Through Glycan Profiling and Analysis
  • From Bench to Bedside: The Role of Glycoproteomic Analysis in Next-Generation Therapeutic Development

Recent Comments

    Archives

    • May 2025
    • April 2025
    • December 2022
    • April 2022
    • November 2021
    • June 2021

    Categories

    • anti venus antibody
    • Blog
    • DNA
    • Elisa Kits
    • estain
    • ly6g antibody
    • Plasmid
    • Product
    • Prsetb
    • psf3
    • sepharose column
    • Uncategorized
    • western blotting blocking
    • wst1 reagent

    Meta

    • Log in
    • Entries feed
    • Comments feed
    • WordPress.org
    © 2025 Entigen Corporation San Jose, CA | Powered by Superbs Personal Blog theme