Abstract
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is out there in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000).

ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply. It has a robust mild emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods accommodates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.
Arachis hypogaea lectin (PNA |
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05-0116-50 | Medicago | 50 mg | EUR 376.8 |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
Asprosin Polyclonal Antibody (Human), HRP |
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4-PAA332Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with HRP. |
Vinculin (VCL) Polyclonal Antibody (Human), HRP |
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4-CAB839Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP. |
Catalase (CAT) Monoclonal Antibody (Human), HRP |
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4-MAC418Hu22-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP. |
Catalase (CAT) Monoclonal Antibody (Human), HRP |
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4-MAC418Hu23-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP. |
Ferroportin (FPN) Monoclonal Antibody (Human), HRP |
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4-MAC489Hu24-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Ferroportin (FPN). This antibody is labeled with HRP. |
Reelin (RELN) Monoclonal Antibody (Human), HRP |
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4-MAC775Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Reelin (RELN). This antibody is labeled with HRP. |
Reelin (RL) Monoclonal Antibody (Rat), HRP |
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4-MAC775Ra21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Reelin (RL). This antibody is labeled with HRP. |
Serglycin (SRGN) Monoclonal Antibody (Human), HRP |
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4-MAC869Hu22-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Serglycin (SRGN). This antibody is labeled with HRP. |
Supervillin (SVIL) Monoclonal Antibody (Human), HRP |
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4-MAC881Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Supervillin (SVIL). This antibody is labeled with HRP. |
Mesothelin (MSLN) Monoclonal Antibody (Human), HRP |
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4-MAH615Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Mesothelin (MSLN). This antibody is labeled with HRP. |
Klotho (KL) Monoclonal Antibody (Human), HRP |
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4-MAH757Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Klotho (KL). This antibody is labeled with HRP. |
Klotho (KL) Monoclonal Antibody (Rat), HRP |
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4-MAH757Ra21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Klotho (KL). This antibody is labeled with HRP. |
Biglycan (BGN) Monoclonal Antibody (Rat), HRP |
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4-MAJ226Ra21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Biglycan (BGN). This antibody is labeled with HRP. |
Calprotectin (CALPRO) Monoclonal Antibody (Human), HRP |
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4-MAK504Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Calprotectin (CALPRO). This antibody is labeled with HRP. |
Amphiregulin (AREG) Polyclonal Antibody (Bovine), HRP |
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4-PAA006Bo01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Amphiregulin (AREG). This antibody is labeled with HRP. |
Amphiregulin (AREG) Polyclonal Antibody (Rat), HRP |
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4-PAA006Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Amphiregulin (AREG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Bovine), HRP |
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4-PAA007Bo01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Angiogenin (ANG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Human), HRP |
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4-PAA007Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP |
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4-PAA007Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP |
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4-PAA007Mu02-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Pig), HRP |
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4-PAA007Po01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Angiogenin (ANG). This antibody is labeled with HRP. |
Angiogenin (ANG) Polyclonal Antibody (Rat), HRP |
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4-PAA007Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Angiogenin (ANG). This antibody is labeled with HRP. |
Cortisone (COR) Polyclonal Antibody (General), HRP |
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4-PAA067Ge01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Cortisone (COR). This antibody is labeled with HRP. |
Androsterone (ADT) Polyclonal Antibody (General), HRP |
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4-PAA070Ge01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Androsterone (ADT). This antibody is labeled with HRP. |
Leptin (LEP) Polyclonal Antibody (Bovine), HRP |
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4-PAA084Bo01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Leptin (LEP). This antibody is labeled with HRP. |
Leptin (LEP) Polyclonal Antibody (Horse), HRP |
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4-PAA084Eq01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Horse Leptin (LEP). This antibody is labeled with HRP. |
Leptin (LEP) Polyclonal Antibody (Mouse), HRP |
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4-PAA084Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Leptin (LEP). This antibody is labeled with HRP. |
Leptin (LEP) Polyclonal Antibody (Rat), HRP |
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4-PAA084Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Leptin (LEP). This antibody is labeled with HRP. |