HRP Conjugated Arachis – Entigen Corporation San Jose, CA

Abstract

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is out there in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000).

ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply. It has a robust mild emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods accommodates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.

Software program

Widespread Western Blot Protocol:

Glycoprotein sample dimension: 500ng
Lectin Focus: 0.1ug/ml

Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS net web page gel
Change gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. The lectin could be utilized to inform aside between human lymphocyte subsets.

Unit Definition

One unit will sort 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily sort

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea lectin (PNA
05-0116-50	Medicago	50 mg	EUR 376.8

Arachis hypogaea Arachin Ahy-3
1-CSB-EP737391ANE	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3
1-CSB-YP737391ANE	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-YP331779ANE(A4)	Cusabio	EUR 703.20 EUR 358.80 EUR 2606.40 EUR 1080.00 EUR 1730.40 EUR 458.40	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P41B
1-CSB-CF337356ANE	Cusabio	EUR 1158.00 EUR 798.00 EUR 858.00	1MG 200ug 500ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE(A4)	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Asprosin Polyclonal Antibody (Human), HRP
4-PAA332Hu01-HRP	Cloud-Clone	EUR 475.20 EUR 4801.20 EUR 1312.80 EUR 614.40 EUR 289.20	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with HRP.

Vinculin (VCL) Polyclonal Antibody (Human), HRP
4-CAB839Hu01-HRP	Cloud-Clone	EUR 345.60 EUR 2958.00 EUR 852.00 EUR 430.80 EUR 234.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP.

Catalase (CAT) Monoclonal Antibody (Human), HRP
4-MAC418Hu22-HRP	Cloud-Clone	EUR 345.60 EUR 2958.00 EUR 852.00 EUR 430.80 EUR 234.00	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP.

Catalase (CAT) Monoclonal Antibody (Human), HRP
4-MAC418Hu23-HRP	Cloud-Clone	EUR 298.80 EUR 2293.20 EUR 685.20 EUR 363.60 EUR 213.60	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP.

Ferroportin (FPN) Monoclonal Antibody (Human), HRP
4-MAC489Hu24-HRP	Cloud-Clone	EUR 386.40 EUR 3537.60 EUR 996.00 EUR 488.40 EUR 252.00	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Ferroportin (FPN). This antibody is labeled with HRP.

Reelin (RELN) Monoclonal Antibody (Human), HRP
4-MAC775Hu21-HRP	Cloud-Clone	EUR 396.00 EUR 3668.40 EUR 1029.60 EUR 501.60 EUR 254.40	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Reelin (RELN). This antibody is labeled with HRP.

Reelin (RL) Monoclonal Antibody (Rat), HRP
4-MAC775Ra21-HRP	Cloud-Clone	EUR 411.60 EUR 3894.00 EUR 1086.00 EUR 524.40 EUR 261.60	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Rat Reelin (RL). This antibody is labeled with HRP.

Serglycin (SRGN) Monoclonal Antibody (Human), HRP
4-MAC869Hu22-HRP	Cloud-Clone	EUR 392.40 EUR 3613.20 EUR 1015.20 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Serglycin (SRGN). This antibody is labeled with HRP.

Supervillin (SVIL) Monoclonal Antibody (Human), HRP
4-MAC881Hu21-HRP	Cloud-Clone	EUR 392.40 EUR 3613.20 EUR 1015.20 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Supervillin (SVIL). This antibody is labeled with HRP.

Mesothelin (MSLN) Monoclonal Antibody (Human), HRP
4-MAH615Hu21-HRP	Cloud-Clone	EUR 392.40 EUR 3613.20 EUR 1015.20 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Mesothelin (MSLN). This antibody is labeled with HRP.

Klotho (KL) Monoclonal Antibody (Human), HRP
4-MAH757Hu21-HRP	Cloud-Clone	EUR 392.40 EUR 3613.20 EUR 1015.20 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Klotho (KL). This antibody is labeled with HRP.

Klotho (KL) Monoclonal Antibody (Rat), HRP
4-MAH757Ra21-HRP	Cloud-Clone	EUR 411.60 EUR 3894.00 EUR 1086.00 EUR 524.40 EUR 261.60	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Rat Klotho (KL). This antibody is labeled with HRP.

Biglycan (BGN) Monoclonal Antibody (Rat), HRP
4-MAJ226Ra21-HRP	Cloud-Clone	EUR 411.60 EUR 3894.00 EUR 1086.00 EUR 524.40 EUR 261.60	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Rat Biglycan (BGN). This antibody is labeled with HRP.

Calprotectin (CALPRO) Monoclonal Antibody (Human), HRP
4-MAK504Hu21-HRP	Cloud-Clone	EUR 477.60 EUR 4830.00 EUR 1320.00 EUR 618.00 EUR 290.40	100ul 10ml 1ml 200ul 20ul

Description: A Mouse monoclonal antibody against Human Calprotectin (CALPRO). This antibody is labeled with HRP.

Amphiregulin (AREG) Polyclonal Antibody (Bovine), HRP
4-PAA006Bo01-HRP	Cloud-Clone	EUR 397.20 EUR 3687.60 EUR 1034.40 EUR 502.80 EUR 255.60	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Bovine Amphiregulin (AREG). This antibody is labeled with HRP.

Amphiregulin (AREG) Polyclonal Antibody (Rat), HRP
4-PAA006Ra01-HRP	Cloud-Clone	EUR 373.20 EUR 3350.40 EUR 949.20 EUR 469.20 EUR 246.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Rat Amphiregulin (AREG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Bovine), HRP
4-PAA007Bo01-HRP	Cloud-Clone	EUR 397.20 EUR 3687.60 EUR 1034.40 EUR 502.80 EUR 255.60	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Bovine Angiogenin (ANG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Human), HRP
4-PAA007Hu01-HRP	Cloud-Clone	EUR 296.40 EUR 2246.40 EUR 673.20 EUR 358.80 EUR 212.40	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Human Angiogenin (ANG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP
4-PAA007Mu01-HRP	Cloud-Clone	EUR 362.40 EUR 3182.40 EUR 907.20 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP
4-PAA007Mu02-HRP	Cloud-Clone	EUR 362.40 EUR 3182.40 EUR 907.20 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Mouse Angiogenin (ANG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Pig), HRP
4-PAA007Po01-HRP	Cloud-Clone	EUR 397.20 EUR 3687.60 EUR 1034.40 EUR 502.80 EUR 255.60	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Pig Angiogenin (ANG). This antibody is labeled with HRP.

Angiogenin (ANG) Polyclonal Antibody (Rat), HRP
4-PAA007Ra01-HRP	Cloud-Clone	EUR 373.20 EUR 3350.40 EUR 949.20 EUR 469.20 EUR 246.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Rat Angiogenin (ANG). This antibody is labeled with HRP.

Cortisone (COR) Polyclonal Antibody (General), HRP
4-PAA067Ge01-HRP	Cloud-Clone	EUR 349.20 EUR 3013.20 EUR 865.20 EUR 435.60 EUR 235.20	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against General Cortisone (COR). This antibody is labeled with HRP.

Androsterone (ADT) Polyclonal Antibody (General), HRP
4-PAA070Ge01-HRP	Cloud-Clone	EUR 349.20 EUR 3013.20 EUR 865.20 EUR 435.60 EUR 235.20	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against General Androsterone (ADT). This antibody is labeled with HRP.

Leptin (LEP) Polyclonal Antibody (Bovine), HRP
4-PAA084Bo01-HRP	Cloud-Clone	EUR 397.20 EUR 3687.60 EUR 1034.40 EUR 502.80 EUR 255.60	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Bovine Leptin (LEP). This antibody is labeled with HRP.

Leptin (LEP) Polyclonal Antibody (Horse), HRP
4-PAA084Eq01-HRP	Cloud-Clone	EUR 404.40 EUR 3781.20 EUR 1057.20 EUR 512.40 EUR 258.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Horse Leptin (LEP). This antibody is labeled with HRP.

Leptin (LEP) Polyclonal Antibody (Mouse), HRP
4-PAA084Mu01-HRP	Cloud-Clone	EUR 362.40 EUR 3182.40 EUR 907.20 EUR 452.40 EUR 240.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Mouse Leptin (LEP). This antibody is labeled with HRP.

Leptin (LEP) Polyclonal Antibody (Rat), HRP
4-PAA084Ra01-HRP	Cloud-Clone	EUR 373.20 EUR 3350.40 EUR 949.20 EUR 469.20 EUR 246.00	100ul 10ml 1ml 200ul 20ul

Description: A Rabbit polyclonal antibody against Rat Leptin (LEP). This antibody is labeled with HRP.

Abstract

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Software program

Biochem/Physiol Actions

Unit Definition

Bodily sort

Preparation Discover

Arachis hypogaea lectin (PNA

Arachis hypogaea Arachin Ahy-3

Arachis hypogaea Arachin Ahy-3

Arachis hypogaea Allergen Ara h 1, clone P17

Arachis hypogaea Allergen Ara h 1, clone P41B

Arachis hypogaea Allergen Ara h 1, clone P17

Arachis hypogaea Allergen Ara h 1, clone P17

Asprosin Polyclonal Antibody (Human), HRP

Vinculin (VCL) Polyclonal Antibody (Human), HRP

Catalase (CAT) Monoclonal Antibody (Human), HRP

Catalase (CAT) Monoclonal Antibody (Human), HRP

Ferroportin (FPN) Monoclonal Antibody (Human), HRP

Reelin (RELN) Monoclonal Antibody (Human), HRP

Reelin (RL) Monoclonal Antibody (Rat), HRP

Serglycin (SRGN) Monoclonal Antibody (Human), HRP

Supervillin (SVIL) Monoclonal Antibody (Human), HRP

Mesothelin (MSLN) Monoclonal Antibody (Human), HRP

Klotho (KL) Monoclonal Antibody (Human), HRP

Klotho (KL) Monoclonal Antibody (Rat), HRP

Biglycan (BGN) Monoclonal Antibody (Rat), HRP

Calprotectin (CALPRO) Monoclonal Antibody (Human), HRP

Amphiregulin (AREG) Polyclonal Antibody (Bovine), HRP

Amphiregulin (AREG) Polyclonal Antibody (Rat), HRP

Angiogenin (ANG) Polyclonal Antibody (Bovine), HRP

Angiogenin (ANG) Polyclonal Antibody (Human), HRP

Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP

Angiogenin (ANG) Polyclonal Antibody (Mouse), HRP

Angiogenin (ANG) Polyclonal Antibody (Pig), HRP

Angiogenin (ANG) Polyclonal Antibody (Rat), HRP

Cortisone (COR) Polyclonal Antibody (General), HRP

Androsterone (ADT) Polyclonal Antibody (General), HRP

Leptin (LEP) Polyclonal Antibody (Bovine), HRP

Leptin (LEP) Polyclonal Antibody (Horse), HRP

Leptin (LEP) Polyclonal Antibody (Mouse), HRP

Leptin (LEP) Polyclonal Antibody (Rat), HRP

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