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HRP Conjugated Arachis

HRP Conjugated Arachis

Posted on November 25, 2021 by Zachary

Abstract

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is out there in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000).

arachis eylabs
arachis eylabs

ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply. It has a robust mild emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods accommodates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.

 

 

Software program

Widespread Western Blot Protocol:

  • Glycoprotein sample dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS net web page gel
  3. Change gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. The lectin could be utilized to inform aside between human lymphocyte subsets.

Unit Definition

One unit will sort 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily sort

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-05mg MyBiosource 0.5mg
EUR 505

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-1mg MyBiosource 1mg
EUR 895

Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

MBS655859-5x1mg MyBiosource 5x1mg
EUR 3885

Arachis hypogaea Conglutin-7

1-CSB-EP757666ANE Cusabio
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  • 10ug
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  • 1MG
Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli

Arachis hypogaea lectin (PNA)

05-0116-10 Medicago 10 mg
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Arachis hypogaea lectin (PNA

05-0116-1000 Medicago 1 g Ask for price

Arachis hypogaea lectin (PNA

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EUR 376.8

Arachis hypogaea 2S protein

MBS717441-10mg MyBiosource 10mg
EUR 1360

Arachis hypogaea 2S protein

MBS717441-1mg MyBiosource 1mg
EUR 230

Arachis hypogaea 2S protein

MBS717441-5x10mg MyBiosource 5x10mg
EUR 6075

rAra h 8 (Arachis hypogaea 8.0101)

10-P210-C100 ExBio 0.1mg
EUR 242

rAra h 9 (Arachis hypogaea 9.0101)

10-P232-C100 ExBio 0.1mg
EUR 242

rAra h 1 (Arachis hypogaea 1.0101)

10-P202-C100 ExBio 0.1mg
EUR 242

rAra h 2 (Arachis hypogaea 2.0201)

10-P208-C100 ExBio 0.1mg
EUR 242

rAra h 1 (Arachis hypogaea 1.0101)

MBS1801756-01mg MyBiosource 0.1mg
EUR 500

rAra h 1 (Arachis hypogaea 1.0101)

MBS1801756-5x01mg MyBiosource 5x0.1mg
EUR 2000

rAra h 8 (Arachis hypogaea 8.0101)

MBS1801762-01mg MyBiosource 0.1mg
EUR 500

rAra h 8 (Arachis hypogaea 8.0101)

MBS1801762-5x01mg MyBiosource 5x0.1mg
EUR 2000

rAra h 2 (Arachis hypogaea 2.0201)

MBS1802242-01mg MyBiosource 0.1mg
EUR 500

rAra h 2 (Arachis hypogaea 2.0201)

MBS1802242-5x01mg MyBiosource 5x0.1mg
EUR 2000

rAra h 9 (Arachis hypogaea 9.0101)

MBS1802243-01mg MyBiosource 0.1mg
EUR 500

rAra h 9 (Arachis hypogaea 9.0101)

MBS1802243-5x01mg MyBiosource 5x0.1mg
EUR 2000

Recombinant Arachis hypogaea Conglutin

CSB-EP720709ANE Cusabio 3989 mg Ask for price

Recombinant Arachis hypogaea Conglutin

CSB-EP720709ANE-20ug Cusabio 20ug Ask for price
Description: 22-145aa

Recombinant Arachis hypogaea Conglutin

AP73786 SAB 1mg
EUR 2826

Recombinant Arachis hypogaea Conglutin

MBS9423366-002mg MyBiosource 0.02mg
EUR 460

Recombinant Arachis hypogaea Conglutin

MBS9423366-01mg MyBiosource 0.1mg
EUR 795

Recombinant Arachis hypogaea Conglutin

MBS9423366-1mg MyBiosource 1mg
EUR 2525

Recombinant Arachis hypogaea Conglutin

MBS9423366-5x1mg MyBiosource 5x1mg
EUR 11205

Arachis hypogaea Arachin Ahy-3

1-CSB-EP737391ANE Cusabio
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  • 1MG
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-YP737391ANE Cusabio
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Lectin (PNA) - HRP (Horseradish Peroxidase)

21510927-1 Bio-WORLD -
EUR 175.57

Arachis hypogaea Lectin (PNA) - Cy3

21761088-1 Bio-WORLD -
EUR 189.93

Arachis hypogaea Lectin (PNA) - Cy3

21761088-2 Bio-WORLD -
EUR 258.72

Arachis hypogaea Lectin (PNA) - Cy5

21761089-1 Bio-WORLD -
EUR 189.93
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