Catalog #: 0540-002
Lot #: 1710006
Purified Recombinant Staphylococcus aureus Panton-Valentine leucocidin (PVL) subunit F (LukF-PV). The theoretical molecular weight of the protein is 34,580 Dalton.
2-3 weeks at -20 ° C, -80 ° C long term
100 µg of protein is supplied in sodium phosphate buffer containing sodium chloride and 5% glycerol, at a concentration of 1,624 mg/ml.
Relevance: this protein can be used in the functional toxicity of PVL assays in combination with Luks-PV, or as a control protein in ELISA or Western Blot assays when detecting LukF in PVL (+) strains of S. aureus.
ELISA: Assay-dependent dilution.
WB: assay dependent dilution; Internal quality control demonstrates detection of 100 ng of LukF-PV protein when detected with polyclonal anti-PVL LukF antibody (cat # 0312-002) in Western drying.
PVL Cytotoxicity Assay: Cytotoxicity can be detected in humans neutrophils when used in combination with Luks-PV (cat # 0540-001) in a concentration range of 1-100 nM.