Catalog #: 0540-001
Lot number: 2001002
Recombinant Staphylococcus, purified and unlabeled aureus Panton-Valentine Leucocidin (PVL) Subunit S (LukS-PV). The theoretical molecular weight of the protein is 32,465 Daltons.
Storage: 2-3 weeks at -20 ° C, -80 ° C long term
Size: 100 µg of protein in PBS plus 5% glycerol is supplied to the concentration of 2.607 mg / mL. The protein demonstrates a molecular weight of approximately 35 kDa.
Relevance: this protein can be used in the functional toxicity of PVL assays in combination with LukF-PV, or as a control protein in ELISA or Western Blot assays when detecting LukS in PVL (+) strains of S. aureus.
ELISA: Assay-dependent dilution.
WB: assay-dependent dilution; Internal quality control demonstrates detection of 50 ng of LukS-PV protein when detected with anti-PVL Luk’s polyclonal antibody.
PVL Cytotoxicity Assay: Cytotoxicity can be detected in humans neutrophils when used in combination with LukF-PV in a concentration range of 1-100 nM.