Skip to content

Entigen Corporation San Jose, CA

Microarray Signarray mRNA library, cancer array

Menu
  • Home
  • Product
  • Elisa Kits
  • DNA
  • Distributors
  • Blog
Menu

SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO).

Posted on April 22, 2022April 22, 2022 by Zachary

Rabies infection (RABV), logical name Rabies lyssavirus, is a dangerous neurotropic infection that causes rabies in people and creatures. Rabies infection has a very wide host range and its transmission most frequently happen through the salivation of creatures. Without mediation preceding illness movement, rabies has the most noteworthy case casualty of any irresistible infection.

RABV contains a solitary abandoned negative-sense RNA genome that encodes five primary proteins: nucleoprotein (N), phosphoprotein (P), network protein (M), glycoprotein (G), and RNA-subordinate RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a vital player interceding infection passage and the significant objective of killing antibodies, accordingly a critical component for immunization and medication plan.

SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO)


SARS-CoV-2 trimeric spike glycoprotein is produced in CHO cells to high return and immaculateness. Protein has proline replacements at deposits 986 and 987, a GSAS replacement at the furin cleavage site (buildups 682-685), and a C-terminal His-tag. HRP formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Spike Glycoprotein (Full-Length), His-Tag (CHO)


Recombinant full-length SARS-CoV-2 spike glycoprotein, produced in Chinese hamster ovary (CHO) cells to high return and immaculateness. The furin cleavage site (amino acids 682-685) has been transformed from RRAR to SRAS and the transmembrane area and intravirion part supplanted with a glycine-serine linker and a His-tag. Horseradish peroxidase formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Trimeric spike CHO

Trimeric SARS-CoV-2 spike protein in prefusion affirmation. C-terminal transmembrane area is supplanted with a trimerization space and a polyhistidine tag. Contains two balancing out proline changes and mixed S1/S2 furin cleavage site (Wrapp et al.

Undeniable level articulation of the monomeric SARS-CoV-2 S protein RBD 320-537 in steadily transfected CHO cells by the EEF1A1-based plasmid vector

The spike (S) protein is one of the three proteins shaping the Covids’ viral envelope. The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial design like the S proteins of other mammalian Covids, with the exception of a novel receptor-restricting area (RBD), which is a critical inducer of host insusceptible reaction. Recombinant SARS-CoV-2 RBD is broadly utilized as a profoundly unambiguous negligible antigen for serological tests.

Right openness of antigenic determinants fundamentally affects the precision of such tests-the antigen must be accurately collapsed, contain no possibly antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation design like the local S protein. In view of the recently evolved p1.1 vector, containing the administrative successions of the Eukaryotic interpretation extension factor 1 alpha quality (EEF1A1) from Chinese hamster, we made two articulation builds encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine labels. RBDv1 contained a local viral sign peptide, RBDv2 – human tPA signal peptide. We transfected a CHO DG44 cell line, chose steadily transfected cells, and played out a couple of rounds of methotrexate-driven intensification of the hereditary tape in the genome.

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1531-10 Biovision 10 µg 235.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1531-50 Biovision 50 µg 709.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1555-10 Biovision 10μg 235.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1555-50 Biovision 50μg 709.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD), Recombinant

P1530-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD), Recombinant

P1530-50 Biovision 50 µg 709.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD 310-568), Recombinant

P1543-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD 310-568), Recombinant

P1543-50 Biovision 50 µg 576 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD; 331-524), Recombinant

P1544-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD; 331-524), Recombinant

P1544-50 Biovision 50 µg 576 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1; His-tag), Recombinant

P1532-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1; His-tag), Recombinant

P1532-50 Biovision 50 µg 661.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2), Recombinant

P1547-10 Biovision 10 μg 235.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2), Recombinant

P1547-50 Biovision 50 μg 782.4 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; S1), Recombinant

P1524-10 Biovision 10 µg 332.4 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; S2), Recombinant

P1525-10 Biovision 10 µg 332.4 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; RBD), Recombinant

P1529-10 Biovision 10 µg 235.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; RBD), Recombinant

P1529-50 Biovision 50 µg 709.2 EUR

SARS-CoV Spike Protein

abx060655-1mg Abbexa 1 mg 2030.4 EUR

Human CellExp™ Coronavirus Spike Protein (MERS-CoV S1; 18-725), Recombinant

P1513-10 Biovision 10 µg 272.4 EUR

Human CellExp™ Coronavirus Spike Protein (MERS-CoV S1; 18-725), Recombinant

P1513-50 Biovision 50 µg 1024.8 EUR

SARS-CoV spike protein Antibody

abx023139-100ug Abbexa 100 ug 1028.4 EUR

SARS-CoV spike protein Antibody

abx023143-100ug Abbexa 100 ug 1028.4 EUR

SARS CoV-2 full length spike protein nanodisc complex

21-817 ProSci 0.025 mg 1968 EUR

SARS CoV-2 full length spike protein in LMNG detergent

21-815 ProSci 0.1 mg 1413.6 EUR

SARS CoV-2 full length spike protein in DIBMA Glycerol

21-816 ProSci 0.025 mg 1703.4 EUR

SARS-CoV-2(COVID-19) Spike Recombinant Protein

10-411 ProSci 0.1 mg 714.3 EUR

SARS-CoV-2 (COVID-19) Spike Recombinant Protein

11-073 ProSci 0.1 mg 695.4 EUR

SARS-CoV-2 (COVID-19) Spike Recombinant Protein

20-233 ProSci 0.1 mg 726.9 EUR

Human CellExp™ SARS-CoV-2 Nucleoprotein, Recombinant

P1554-10 Biovision 10 µg 211.2 EUR

Human CellExp™ SARS-CoV-2 Nucleoprotein, Recombinant

P1554-50 Biovision 50 µg 661.2 EUR

SARS-CoV/ SARS-CoV-2 (COVID-19) spike antibody [1A9]

BSV-COV-AB-01 BioServUK 25 ul Ask for price

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-107 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-109 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-111 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-118 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-207 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-209 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

10-300 ProSci 0.1 mg 632.4 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

21-805 ProSci 50 ug 468.6 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

21-807 ProSci 50 ug 437.1 EUR

SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein

10-100 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein

10-117 ProSci 0.1 mg 752.1 EUR

SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein

10-204 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein

10-206 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein

10-303 ProSci 0.1 mg 632.4 EUR

SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein

10-008 ProSci 0.1 mg 714.3 EUR

SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein

10-015 ProSci 0.1 mg 714.3 EUR

Sars-Cov, Spike (Middle) Recom Protein

abx060656-1mg Abbexa 1 mg 2030.4 EUR

Recombinant Coronavirus Spike Protein (SARS-CoV-2; ECD)

P1533-10 Biovision 10 µg 235.2 EUR

Recombinant Coronavirus Spike Protein (SARS-CoV-2; ECD)

P1533-50 Biovision 50 µg 709.2 EUR

Recombinant SARS-CoV-2 Spike Protein S1 (Fc tag)

P1541-10 Biovision 10 µg 211.2 EUR

Recombinant SARS-CoV-2 Spike Protein S1 (Fc tag)

P1541-50 Biovision 50 µg 818.4 EUR

SARS-CoV-2 Spike Peptide

9083P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9087P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9091P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9095P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein

10-115 ProSci 0.1 mg 651.3 EUR

Recombinant SARS-CoV-2 Spike Protein S1 (His-tag)

P1540-10 Biovision 10 µg 211.2 EUR

Recombinant SARS-CoV-2 Spike Protein S1 (His-tag)

P1540-50 Biovision 50 µg 818.4 EUR

SARS-CoV-2 (COVID-19) Spike RBD + SD1 Recombinant Protein

10-304 ProSci 0.1 mg 632.4 EUR

GENLISA™ Human SARS-CoV-2 (Covid-19) Spike Protein Antigen Quantitative ELISA

KBVH015-10 Krishgen 12 × 8 wells 1642.5 EUR

Spike (SARS-CoV-2) Lentivirus

78010-1 BPS Bioscience 100 µl 835 EUR

Spike (SARS-CoV-2) Lentivirus

78010-2 BPS Bioscience 500 µl x 2 2095 EUR

*SARS-CoV-2 Spike Protein RBD Recombinant HIS Labeled

ICOV2RBDRHIS100UG Innovative research each 544 EUR

*SARS-CoV-2 Spike Protein RBD Recombinant HIS Labeled

ICOV2RBDRHIS50UG Innovative research each 338 EUR

SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein (biotin)

21-806 ProSci 50 ug 437.1 EUR

SARS-CoV-2 (COVID-19) Spike S Trimer Recombinant Protein

20-182 ProSci 0.1 mg 651.3 EUR

SARS-CoV-2 (COVID-19) Spike E Mosaic Recombinant protein

39-114 ProSci 0.05 mg 556.8 EUR

SARS-CoV-2 (COVID-19) Spike Antibody

3525-002mg ProSci 0.02 mg 206.18 EUR

SARS-CoV-2 (COVID-19) Spike Antibody

3525-01mg ProSci 0.1 mg 523.7 EUR

SARS-CoV-2 (COVID-19) spike antibody

BSV-COV-AB-05 BioServUK 25 ul Ask for price

SARS-CoV-2 (COVID-19) spike antibody

BSV-COV-AB-06 BioServUK 100 ul Ask for price

SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, Fc Tag)

BSV-COV-PR-09 BioServUK 100 ug Ask for price

SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)

BSV-COV-PR-01 BioServUK 100 ug Ask for price

SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)

BSV-COV-PR-08 BioServUK 100 ug Ask for price

SARS-CoV-2 (COVID-19) Spike RBD domain Recombinant Protein

20-232 ProSci 0.1 mg 726.9 EUR

SARS-CoV-2 (COVID-19) Spike Recombinant protein (800-1000 aa)

39-125 ProSci 0.05 mg 556.8 EUR

SARS-CoV-2 (COVID-19) Spike Recombinant protein (1000-1200 aa)

39-126 ProSci 0.05 mg 556.8 EUR

SARS-CoV/ SARS-CoV-2 (COVID-19) spike antibody [1A9] 100 ul

BSV-COV-AB-02 BioServUK 100 ul Ask for price

Sars-Cov, Spike (N-Term) Recom Protein

abx060657-1mg Abbexa 1 mg 2247.6 EUR

SARS-CoV-2 (COVID-19) Trimeric Spike (S) Recombinant Protein

10-075 ProSci 0.1 mg 991.5 EUR

SARS-CoV-2 (COVID-19) Variant Spike Protein RBD (E484D) Recombinant Protein

21-829 ProSci 0.1 mg 714.3 EUR

Recombinant Coronavirus Spike Protein (SARS-CoV S2)

P1519-10 Biovision 10µg 187.2 EUR
×

For the RBDv2 variation, a high return clonal maker cell line was gotten. We fostered a straightforward purging plan that reliably yielded up to 30 mg of RBD protein per liter of the basic shake flagon cell culture. Cleaned proteins were dissected by polyacrylamide gel electrophoresis in lessening and non-diminishing circumstances and gel filtration; for RBDv2 protein, the monomeric structure content surpassed 90% for a very long time. Deglycosylation with PNGase F and mass spectrometry affirmed the presence of N-glycosylation. The antigen delivered by the depicted procedure is reasonable for serological tests and subunit immunization review.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • Compare Appoptosis lab reagents for research
  • Compare recombinant lab reagents for research
  • ELISA Lab Reagents for Research
  • Apo Active PE- Caspase 3
  • Equine IFNg ELISA Kit.

Recent Comments

    Archives

    • December 2022
    • April 2022
    • November 2021
    • June 2021

    Categories

    • anti venus antibody
    • Blog
    • DNA
    • Elisa Kits
    • estain
    • ly6g antibody
    • Plasmid
    • Product
    • Prsetb
    • psf3
    • sepharose column
    • Uncategorized
    • western blotting blocking
    • wst1 reagent

    Meta

    • Log in
    • Entries feed
    • Comments feed
    • WordPress.org
    © 2023 Entigen Corporation San Jose, CA | Powered by Superbs Personal Blog theme