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SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO).

Posted on April 22, 2022April 22, 2022 by Zachary

Rabies infection (RABV), logical name Rabies lyssavirus, is a dangerous neurotropic infection that causes rabies in people and creatures. Rabies infection has a very wide host range and its transmission most frequently happen through the salivation of creatures. Without mediation preceding illness movement, rabies has the most noteworthy case casualty of any irresistible infection.

RABV contains a solitary abandoned negative-sense RNA genome that encodes five primary proteins: nucleoprotein (N), phosphoprotein (P), network protein (M), glycoprotein (G), and RNA-subordinate RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a vital player interceding infection passage and the significant objective of killing antibodies, accordingly a critical component for immunization and medication plan.

SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO)


SARS-CoV-2 trimeric spike glycoprotein is produced in CHO cells to high return and immaculateness. Protein has proline replacements at deposits 986 and 987, a GSAS replacement at the furin cleavage site (buildups 682-685), and a C-terminal His-tag. HRP formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Spike Glycoprotein (Full-Length), His-Tag (CHO)


Recombinant full-length SARS-CoV-2 spike glycoprotein, produced in Chinese hamster ovary (CHO) cells to high return and immaculateness. The furin cleavage site (amino acids 682-685) has been transformed from RRAR to SRAS and the transmembrane area and intravirion part supplanted with a glycine-serine linker and a His-tag. Horseradish peroxidase formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Trimeric spike CHO

Trimeric SARS-CoV-2 spike protein in prefusion affirmation. C-terminal transmembrane area is supplanted with a trimerization space and a polyhistidine tag. Contains two balancing out proline changes and mixed S1/S2 furin cleavage site (Wrapp et al.

Undeniable level articulation of the monomeric SARS-CoV-2 S protein RBD 320-537 in steadily transfected CHO cells by the EEF1A1-based plasmid vector

The spike (S) protein is one of the three proteins shaping the Covids’ viral envelope. The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial design like the S proteins of other mammalian Covids, with the exception of a novel receptor-restricting area (RBD), which is a critical inducer of host insusceptible reaction. Recombinant SARS-CoV-2 RBD is broadly utilized as a profoundly unambiguous negligible antigen for serological tests.

Right openness of antigenic determinants fundamentally affects the precision of such tests-the antigen must be accurately collapsed, contain no possibly antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation design like the local S protein. In view of the recently evolved p1.1 vector, containing the administrative successions of the Eukaryotic interpretation extension factor 1 alpha quality (EEF1A1) from Chinese hamster, we made two articulation builds encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine labels. RBDv1 contained a local viral sign peptide, RBDv2 – human tPA signal peptide. We transfected a CHO DG44 cell line, chose steadily transfected cells, and played out a couple of rounds of methotrexate-driven intensification of the hereditary tape in the genome.

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P1531-10 Biovision 10 µg 235.2 EUR

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Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1555-10 Biovision 10μg 235.2 EUR

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P1532-50 Biovision 50 µg 661.2 EUR

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abx060655-10g Abbexa 10 µg 1962.5 EUR

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abx060656-10g Abbexa 10 µg 1962.5 EUR

SARS-CoV Spike Protein

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SARS-CoV Spike Protein

abx060657-100g Abbexa 100 µg Ask for price

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abx060657-10g Abbexa 10 µg 1962.5 EUR

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Spike Protein of SARS-CoV-2 (COVID-19)

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MBS434283-5x005mg MyBiosource 5x0.05mg 1580 EUR

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MBS462257-01mg MyBiosource 0.1mg 320 EUR

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MBS462257-5x01mg MyBiosource 5x0.1mg 1390 EUR

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MBS462258-01mg MyBiosource 0.1mg 320 EUR

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MBS462258-5x01mg MyBiosource 5x0.1mg 1390 EUR

SARS-CoV-2 Spike S1 protein

MBS398011-01mg MyBiosource 0.1mg 510 EUR

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MBS398011-5x01mg MyBiosource 5x0.1mg 2225 EUR

SARS-CoV-2 Spike Protein RBD

abx620023-1mg Abbexa 1 mg 287.5 EUR

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abx620024-50g Abbexa 50 µg 287.5 EUR

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For the RBDv2 variation, a high return clonal maker cell line was gotten. We fostered a straightforward purging plan that reliably yielded up to 30 mg of RBD protein per liter of the basic shake flagon cell culture. Cleaned proteins were dissected by polyacrylamide gel electrophoresis in lessening and non-diminishing circumstances and gel filtration; for RBDv2 protein, the monomeric structure content surpassed 90% for a very long time. Deglycosylation with PNGase F and mass spectrometry affirmed the presence of N-glycosylation. The antigen delivered by the depicted procedure is reasonable for serological tests and subunit immunization review.

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