Skip to content

Entigen Corporation San Jose, CA

Microarray Signarray mRNA library, cancer array

Menu
  • Home
  • Product
  • Elisa Kits
  • DNA
  • Distributors
  • Blog
Menu

SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO).

Posted on April 22, 2022April 22, 2022 by Zachary

Rabies infection (RABV), logical name Rabies lyssavirus, is a dangerous neurotropic infection that causes rabies in people and creatures. Rabies infection has a very wide host range and its transmission most frequently happen through the salivation of creatures. Without mediation preceding illness movement, rabies has the most noteworthy case casualty of any irresistible infection.

RABV contains a solitary abandoned negative-sense RNA genome that encodes five primary proteins: nucleoprotein (N), phosphoprotein (P), network protein (M), glycoprotein (G), and RNA-subordinate RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a vital player interceding infection passage and the significant objective of killing antibodies, accordingly a critical component for immunization and medication plan.

SARS-CoV-2 Spike Glycoprotein (Trimeric), His-Tag (CHO)


SARS-CoV-2 trimeric spike glycoprotein is produced in CHO cells to high return and immaculateness. Protein has proline replacements at deposits 986 and 987, a GSAS replacement at the furin cleavage site (buildups 682-685), and a C-terminal His-tag. HRP formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Spike Glycoprotein (Full-Length), His-Tag (CHO)


Recombinant full-length SARS-CoV-2 spike glycoprotein, produced in Chinese hamster ovary (CHO) cells to high return and immaculateness. The furin cleavage site (amino acids 682-685) has been transformed from RRAR to SRAS and the transmembrane area and intravirion part supplanted with a glycine-serine linker and a His-tag. Horseradish peroxidase formed protein additionally accessible. SARS-CoV-2, recently known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 sickness.

SARS-CoV-2 Trimeric spike CHO

Trimeric SARS-CoV-2 spike protein in prefusion affirmation. C-terminal transmembrane area is supplanted with a trimerization space and a polyhistidine tag. Contains two balancing out proline changes and mixed S1/S2 furin cleavage site (Wrapp et al.

Undeniable level articulation of the monomeric SARS-CoV-2 S protein RBD 320-537 in steadily transfected CHO cells by the EEF1A1-based plasmid vector

The spike (S) protein is one of the three proteins shaping the Covids’ viral envelope. The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial design like the S proteins of other mammalian Covids, with the exception of a novel receptor-restricting area (RBD), which is a critical inducer of host insusceptible reaction. Recombinant SARS-CoV-2 RBD is broadly utilized as a profoundly unambiguous negligible antigen for serological tests.

Right openness of antigenic determinants fundamentally affects the precision of such tests-the antigen must be accurately collapsed, contain no possibly antigenic non-vertebrate glycans, and, ideally, ought to have a glycosylation design like the local S protein. In view of the recently evolved p1.1 vector, containing the administrative successions of the Eukaryotic interpretation extension factor 1 alpha quality (EEF1A1) from Chinese hamster, we made two articulation builds encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine labels. RBDv1 contained a local viral sign peptide, RBDv2 – human tPA signal peptide. We transfected a CHO DG44 cell line, chose steadily transfected cells, and played out a couple of rounds of methotrexate-driven intensification of the hereditary tape in the genome.

Spike (SARS-CoV-2) Lentivirus

78010-2 BPS Bioscience 500 µl x 2 2095 EUR

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028 EpiGentek
  • 388.30 EUR
  • 860.20 EUR
  • 20 ul
  • 100 ul

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag

E80024 EpiGentek
  • 635.80 EUR
  • 4995.10 EUR
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion

E80026 EpiGentek
  • 588.50 EUR
  • 823.90 EUR
  • 20 ul
  • 50 ul

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025 EpiGentek
  • 635.80 EUR
  • 3934.70 EUR
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag

E80021 EpiGentek
  • 635.80 EUR
  • 4276.80 EUR
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag

E80023 EpiGentek
  • 635.80 EUR
  • 3934.70 EUR
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag

E80020 EpiGentek
  • 635.80 EUR
  • 4276.80 EUR
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag

E80022 EpiGentek
  • 635.80 EUR
  • 4276.80 EUR
  • 100 ul
  • 1 ml

Spike S1, Fc fusion (SARS-CoV-2)

100688-2 BPS Bioscience 50 µg 505 EUR

Anti-Spike S1 Antibody (SARS-CoV-2)

100715-2 BPS Bioscience 100 µg 440 EUR

Spike S2, Fc-Tag (SARS-CoV-2)

100895-2 BPS Bioscience 500 µg_x000D_ 1815 EUR

Spike S1 RBD, His-tag (SARS-CoV-2)

100687-2 BPS Bioscience 100 µg 520 EUR

Spike S1 RBD, Fc fusion (SARS-CoV-2)

100699-2 BPS Bioscience 100 µg 520 EUR

Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter)

79942-2 BPS Bioscience 500 µl x 2 4405 EUR

Spike (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter)

79981-2 BPS Bioscience 500 µl x 2 5245 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD), Recombinant

P1530-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD), Recombinant

P1530-50 Biovision 50 µg 709.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1531-10 Biovision 10 µg 235.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1531-50 Biovision 50 µg 709.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2), Recombinant

P1547-10 Biovision 10 μg 235.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2), Recombinant

P1547-50 Biovision 50 μg 782.4 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1555-10 Biovision 10μg 235.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

P1555-50 Biovision 50μg 709.2 EUR

Spike S1 RBD-Nucleocapsid Protein Chimera (SARS-CoV-2)

100938-2 BPS Bioscience 50 µg 555 EUR

SARS-CoV Spike Protein

abx060655-1mg Abbexa 1 mg 2030.4 EUR

SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag

E80027 EpiGentek
  • 635.80 EUR
  • 4087.60 EUR
  • 1 ml
  • 100 ul

SARS-CoV-2 Spike Peptide

9083P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9087P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9091P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Peptide

9095P ProSci 0.05 mg 235.5 EUR

Spike (SARS-CoV-2) Lentivirus

78010-1 BPS Bioscience 100 µl 835 EUR

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028-2 EpiGentek 100 ul 860.2 EUR

Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2)

100684-2 BPS Bioscience 50 µg 435 EUR

Spike S1 RBD, Avi-His-tag (SARS-CoV-2)

100696-2 BPS Bioscience 1 mg 3200 EUR

Spike Trimer (S1+S2), His-tag (SARS-CoV-2)

100728-2 BPS Bioscience 1 mg 2995 EUR

Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (Luc Reporter)

78028-2 BPS Bioscience 500 µl x 2 4510 EUR

Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (eGFP Reporter)

78035-2 BPS Bioscience 500 µl x 2 5145 EUR

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag

E80024-2 EpiGentek 1 ml 4995.1 EUR

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion

E80026-2 EpiGentek 50 ul 823.9 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; S1), Recombinant

P1524-10 Biovision 10 µg 332.4 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; S2), Recombinant

P1525-10 Biovision 10 µg 332.4 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; RBD), Recombinant

P1529-10 Biovision 10 µg 235.2 EUR

Human CellExp™ Coronavirus Spike Protein (SARS-CoV-2; RBD), Recombinant

P1529-50 Biovision 50 µg 709.2 EUR

SARS-CoV Spike Antibody

3219-002mg ProSci 0.02 mg 206.18 EUR

SARS-CoV Spike Antibody

3219-01mg ProSci 0.1 mg 523.7 EUR

SARS-CoV Spike Antibody

3221-002mg ProSci 0.02 mg 206.18 EUR

SARS-CoV Spike Antibody

3221-01mg ProSci 0.1 mg 523.7 EUR

SARS-CoV Spike Antibody

3223-002mg ProSci 0.02 mg 206.18 EUR

SARS-CoV Spike Antibody

3223-01mg ProSci 0.1 mg 523.7 EUR

SARS-CoV Spike Antibody

3225-002mg ProSci 0.02 mg 206.18 EUR

SARS-CoV Spike Antibody

3225-01mg ProSci 0.1 mg 523.7 EUR

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025-2 EpiGentek 1 ml 3934.7 EUR

Spike Trimer (S1+S2), His-tag (SARS-CoV)

100789-2 BPS Bioscience 500 µg_x000D_ 1900 EUR

Spike (SARS-CoV-1) Pseudotyped Lentivirus (Luc Reporter)

78614-2 BPS Bioscience 500 µl x 2 4320 EUR

SARS-CoV spike protein Antibody

abx023139-100ug Abbexa 100 ug 1028.4 EUR

SARS-CoV spike protein Antibody

abx023143-100ug Abbexa 100 ug 1028.4 EUR

Spike S1 RBD, Fc-Fusion, Avi-Tag (SARS-CoV-2)

100698-2 BPS Bioscience 1 mg 2500 EUR

Spike S1 (16-685), Avi-His-tag (SARS-CoV-2)

100730-2 BPS Bioscience 1 mg 2720 EUR

Spike S1 RBD (V367F), Avi-His-tag (SARS-CoV-2)

100769-2 BPS Bioscience 1 mg 2500 EUR

Spike S1 RBD (V483A), Avi-His-tag (SARS-CoV-2)

100846-2 BPS Bioscience 1 mg 2600 EUR

Spike S1 (B.1.351), Avi-His-Tag (SARS-CoV-2)

100992-2 BPS Bioscience 1 mg 2850 EUR

Spike (SARS-CoV-2, UK Variant) Pseudotyped Lentivirus (Luc Reporter)

78112-2 BPS Bioscience 500 µl x 2 4405 EUR

Spike (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter)

78637-2 BPS Bioscience 500 µl x 2 3995 EUR

Spike(SARS-CoV-2) Pseudotyped Lentivirus (Luc-eGFP Dual Reporter)

79982-2 BPS Bioscience 500 µl x 2 8110 EUR

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag

E80021-2 EpiGentek 1 ml 4276.8 EUR

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag

E80023-2 EpiGentek 1 ml 3934.7 EUR

3CL Protease (SARS-CoV-1 / SARS-CoV-2) Substrate

79952-2 BPS Bioscience 10 mg 3460 EUR

SARS-CoV-2 Spike S2 Peptide

9119P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike S2 Peptide

9123P ProSci 0.05 mg 235.5 EUR

SARS-CoV-2 Spike Monoclonal Antibody

A73664-050 EpiGentek 50 ul 341 EUR

SARS-CoV-2 Spike Monoclonal Antibody

A73664-100 EpiGentek 100 ul 518.1 EUR

SARS-CoV-2 Spike RBD Nanobody

A73680-050 EpiGentek 50 ul Ask for price

SARS-CoV-2 Spike RBD Nanobody

A73680-100 EpiGentek 100 ul 882.2 EUR

SARS-CoV-2 Spike Monoclonal Antibody

A73664 EpiGentek
  • 341.00 EUR
  • 518.10 EUR
  • 50 ul
  • 100 ul

SARS-CoV-2 Spike RBD Nanobody

A73680 EpiGentek
  • Ask for price
  • 882.20 EUR
  • 50 ul
  • 100 ul

Human CellExp™ SARS-CoV-2 Spike Protein (S1; His-tag), Recombinant

P1532-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (S1; His-tag), Recombinant

P1532-50 Biovision 50 µg 661.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD 310-568), Recombinant

P1543-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD 310-568), Recombinant

P1543-50 Biovision 50 µg 576 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD; 331-524), Recombinant

P1544-10 Biovision 10 µg 187.2 EUR

Human CellExp™ SARS-CoV-2 Spike Protein (RBD; 331-524), Recombinant

P1544-50 Biovision 50 µg 576 EUR

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag

E80020-2 EpiGentek 1 ml 4276.8 EUR

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag

E80022-2 EpiGentek 1 ml 4276.8 EUR
×

For the RBDv2 variation, a high return clonal maker cell line was gotten. We fostered a straightforward purging plan that reliably yielded up to 30 mg of RBD protein per liter of the basic shake flagon cell culture. Cleaned proteins were dissected by polyacrylamide gel electrophoresis in lessening and non-diminishing circumstances and gel filtration; for RBDv2 protein, the monomeric structure content surpassed 90% for a very long time. Deglycosylation with PNGase F and mass spectrometry affirmed the presence of N-glycosylation. The antigen delivered by the depicted procedure is reasonable for serological tests and subunit immunization review.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • Compare Appoptosis lab reagents for research
  • Compare recombinant lab reagents for research
  • ELISA Lab Reagents for Research
  • Apo Active PE- Caspase 3
  • Equine IFNg ELISA Kit.

Recent Comments

    Archives

    • December 2022
    • April 2022
    • November 2021
    • June 2021

    Categories

    • anti venus antibody
    • Blog
    • DNA
    • Elisa Kits
    • estain
    • ly6g antibody
    • Plasmid
    • Product
    • Prsetb
    • psf3
    • sepharose column
    • Uncategorized
    • western blotting blocking
    • wst1 reagent

    Meta

    • Log in
    • Entries feed
    • Comments feed
    • WordPress.org
    © 2023 Entigen Corporation San Jose, CA | Powered by Superbs Personal Blog theme